Pages 1-15
Cuong, L.V.; Chien, L.H.; Karam, D.S.; Bao, T.Q.; Quy, N.V.; Thanh, N.M.; Hoan, L.N.; & Tam, P.M.
Abstract | Full Text
Abstract
Assessing soil properties across different forest types is a critical aspect of silviculture. This study aimed
to investigate the variations in the characteristics of yellow-brown ferralitic soil beneath the canopy of
tropical semi-moist evergreen closed forests subjected to varying intensities of selective logging in the
Hoa Binh Hydropower Reservoir area, Hoa Binh Province, Vietnam. Twelve 1000 m2 plots representing
rich, medium, poor, and mixed wood–bamboo forests were sampled. At each plot, soil sample was
collected at two depths from five random points along an S-shaped transect, then composited by depth
for physical and chemical analysis. The results showed that: (1) The characteristics of yellow-brown
ferralitic soil varied significantly based on the forest types. Soil water content was higher in the rich
and medium forests compared to the poor and mixed wood-bamboo forests. Soil clay content
progressively increased from the rich forest to the poor forest, with the lowest levels found in the mixed
wood-bamboo forest. Conversely, soil silt content tended to increase with forest degradation, while soil
sand content decreased significantly as degradation intensified. (2) An increase in stand timber volume
is linked to a decrease in soil bulk density and particle density. In contrast, it is associated with higher
levels of soil pHKCl, as well as greater concentrations of soil organic matter, organic carbon and
essential nutrients such as total nitrogen, phosphorus, and potassium. (3) Organic matter content in
yellow-brown ferralitic soil was most strongly influenced by standing tree volume, followed by soil
pHKCl, microbial community, and water content. The findings of this study not only contribute to a
deeper understanding of soil dynamics under the influence of logging but also offer a scientific basis
for the development of silvicultural practices and sustainable forest management strategies.
Keywords: Yellow-brown ferralitic soil, stand, forest types, forest litter, soil microorganisms
Pages 16-27
Amaily Akter; Ali Tan Kee Zuan; Susilawati Binti Kasim; Adibah Mohd Amin; Zakry Fitri Ab Aziz; Md Ekhlasur Rahman; Buraq Musa Sadeq; Abba Nabayi; Rafio Rahmatullah; Shah Iftekhar Ahmed; & Md Habibullah Siddiki
Abstract | Full Text
Abstract
Tomato (Solanum lycopersicum L.) production often relies heavily on chemical nitrogen (N) fertilizers,
raising concerns about costs, sustainability, and soil health. Plant growth-promoting bacteria (PGPB)
represent a promising alternative that may reduce fertilizer inputs while maintaining fruit yield and
quality. This study was undertaken to investigate the influence of two PGPB strains, Bacillus tequilensis
(UPMRB9) and Bacillus subtilis (UPMB10), applied under different N levels on tomato performance and
soil enzymatic activity. A glasshouse experiment was arranged in a completely randomized design with
nine treatments, combining bacterial inoculation and three N application rates (0, 50, and 100% of the
recommended dose). Inoculated plants consistently performed better than the uninoculated control, with
significant improvements in yield attributes (flower number, fruit number, fruit set, and fruit weight) and
fruit quality traits. The combination of UPMRB9 with 50% of the recommended N rate (UPMRB9N50)
produced the greatest effects, increasing fruit yield by 27.27%, lycopene content by 12.16%, antioxidant
activity by 26.96%, and soil microbial activity by 11.14% compared with the full N dose without
inoculation. Higher total soluble solids (5.15 °Brix) were also recorded in UPMRB9N50-treated plants,
reflecting better fruit quality. Overall, the findings suggest that microbial inoculation, particularly with
UPMRB9, can reduce N fertilizer use by almost 50% while still enhancing tomato yield and fruit quality.
Such results provide important preliminary evidence for adopting PGPB as a viable strategy to reduce
chemical fertilizer dependency and promote more sustainable tomato production.
Keywords: PGPB, Nitrogen fertilizer, Antioxidants, Enzyme, PCA
Pages 28-42
Cuong, L.V.; Quy, N.V; Daljit, S.K.; Chau, M.H.; Phu, N.T.; Tien, T.V; Doi, B.T.; Hung, B.M.; Ha, N.T.; Dai, Y.Z.; & Quy, N.V.
Abstract | Full Text
Abstract
Mangroves are highly productive blue carbon (C) ecosystems with an exceptional capacity to store
organic C in soils. Soil organic C storage in these systems is recognized as a key component of the
global C cycle and an important natural mechanism for climate change mitigation. Nonetheless,
knowledge of how soil C dynamics vary with forest development in mangrove plantations remains
limited. To address this gap, the current study quantified changes in soil C stocks along a forest age
sequence of Rhizophora apiculata Blume plantations in the Kien Vang coastal area, Ca Mau Province,
Vietnam, through systematic field surveys. The results revealed that: (1) Soil C content and stocks
significantly increased with the developing forest age. (2) Soil C concentration and storage significantly
decreased with increasing of soil depth along forest age. (3) The stocks of total soil organic C (SCs)
increased from 94.97 Mg C ha-1 to 224.75 Mg C ha-1, during forest development. SCs exhibited
obvious surface aggregation, with more than 70% of SCs occurring in the soil of 0–40 cm depth,
underscoring the pivotal role of surface soils in C sequestration. (4) Based on the random forest
regression analysis, soil salinity, total nitrogen, and stand density were identified as the key factors
regulating variations in SCs across forest age development, accounting for 15.3%, 13.4%, and 11.8%
of the variation, respectively. These findings highlight the vital role of R. apiculata mangrove
plantations as effective C sinks and underscore their contribution to global climate change mitigation
efforts and sustainable forest management strategies for improved soil health.
Keywords: Mangrove plantations, carbon dynamics, climate change mitigation, forest age gradient, soil organic carbon storage, sustainable forest management
Pages 43-51
Udoh, Uyo-obong S.; Ali T.K.Z.; Tan, G.H.; & Nor’aini, A.R.
Abstract | Full Text
Abstract
Rice production has recently declined, primarily due to reduced soil fertility and the high cost of
synthetic fertilizers. This necessitated exploring greener alternatives, such as silicate-solubilizing
bacteria (SSB), which are vital for enhancing soil fertility by making silicate available for plant use.
The objectives of this study are to screen selected bacteria from culture collections for silicate-
solubilizing properties, characterize them for silicate-solubilizing properties, and evaluate the effect of
silicate-solubilizing bacteria on rice growth and yield. The study was laid out in an RCBD with 2 silicate
sources (Calcium Silicate and Kaolinite) and 3 bacterial strains (UPMC1341, UPMC1446, and
UPMC383), with B0 serving as a negative control. An 8-treatment combination and a total of 24
experimental units were supplied with silicates, inoculated with 3 SSBs on day one, and re-inoculated
at 55 days after transplanting (DAP). In the results, bacterial strains, most importantly UPMC1446,
significantly increased plant height and the number of tillers (29 ± 2.08). UPMC1341 also increased
the number of panicles/plant and the number of spikelets/panicle, 36±2.34, 211±2.25,respectively, in
combination with Kaolinite. This research highlighted the efficacy of SSBs, most importantly
UPMC1446 and UPMC1341, in solubilizing insoluble silicate, thereby enhancing rice growth and
yield, particularly when supplied with kaolinite.
Keywords: Silicate-solubilizing bacteria, organic acids, rice yield
Pages 52-68
MDM Ridzuan; DS Karam; Muhammad Firdaus Sulaiman; & A Abdu
Abstract | Full Text
Abstract
This study assessed soil quality differences between five uniformly aged fruit orchards and an adjacent
arboretum reference by applying the Tropical Soil Quality Index (TSQI), which integrates physical,
chemical, and biological indicators. The arboretum, which has remained undisturbed relative to
orchard management, was used as the benchmark for evaluating current soil condition after long term
cultivation in orchard plots. Composite topsoil (0–30 cm) sampling was conducted by dividing each
field into nine areas and combining five auger subsamples per area, with separate handling for
microbial biomass analysis. Measured soil properties included texture, coarse fragments, bulk density,
organic matter proxies (total carbon and total nitrogen), cation exchange capacity, exchangeable bases,
available phosphorus, soil pH, exchangeable aluminium, microbial biomass carbon, and microbial
biomass nitrogen. These were analysed using Analysis of Variance and TSQI scoring. The results
showed clear differences between land uses, with the arboretum exhibiting lower bulk density than
orchard soils. Chemical indicators also distinguished the arboretum, where organic matter related
fertility indicators were higher (organic matter, total carbon, total nitrogen, cation exchange capacity)
than in orchards. The arboretum also had a lower C/N ratio compared to the orchards. Nutrient patterns
varied across sites with available phosphorus lowest in the arboretum while all sites remained within a
deficient range, and exchangeable magnesium sufficient only in the arboretum but low in most orchards,
despite exchangeable potassium being within a sufficient range across plots. Available phosphorus was
lowest in the arboretum, although all sites were phosphorus-deficient. Exchangeable magnesium was
sufficient only in the arboretum, while potassium levels were adequate across all plots. Soil pH and
exchangeable aluminium were similar, with the arboretum showing slightly higher acidity and
aluminium content. Biological indicators showed difference from the organic matter pattern, with
microbial biomass carbon highest in one orchard soil and lowest in the arboretum, while microbial
biomass nitrogen peaked in the arboretum and was lowest in another orchard. Soil quality was further
assessed using the TSQI, which integrated multiple indicators into a single comparative framework.
The arboretum recorded a higher index score (63.64%) than the fruit orchards (50.00%), primarily due
to its greater total carbon, total nitrogen, and exchangeable magnesium, suggesting that rebuilding
organic matter linked nutrient retention and alleviating magnesium limitation are central leverage
points for improving orchard soil function under sustained management. These findings indicate that
long-term conversion of forest land to fruit orchards, combined with intensive management practices,
has gradually improved soil quality, with orchard soils approaching the benchmark condition
represented by the arboretum. The results also confirm that the TSQI is a sensitive and effective tool for
assessing soil condition in tropical agroecosystems and detecting changes driven by management over
time.
Keywords: Arboretum, fruit orchard, soil quality, Tropical Soil Quality Index
Pages 69-87
Japri, Norsyuhada; Yusuf, Nor Azma; Abdullah, Rosazlin; & Mahmood, Noor Zalina
Abstract | Full Text
Abstract
The prolonged overuse of inorganic fertilizers has raised environmental concerns, prompting the
search for alternative nutrient management strategies that can maintain crop productivity.
Vermicompost (VC) and vermicompost leachate (VCL) show promise as organic nutrient sources;
however, their effectiveness under field conditions for medicinal crops remains inadequately
documented. This study evaluated the effects of VC and VCL on the growth performance, soil
properties, and nutrient assimilation of Clinacanthus nutans, a high-value medicinal herb with
antiviral, wound-healing and anticancer agent. A field experiment was conducted using a
randomized complete block design (RCBD) with four treatments: (T1) control; (T2) recommended
rate of chemical fertilizer; (T3) vermicompost (15 t ha-1); and (T4) an integrated treatment combining
50% chemical fertilizer with foliar VCL (2 mL L−1). Results indicated that the full CF treatment
produced the highest biomass, whereas T4 achieved statistically comparable yields despite a 50%
reduction in synthetic fertilizer input. Vermicompost significantly enhanced soil organic carbon and
potassium availability but exhibited a slower nitrogen release. The T4 treatment also reduced soil
alkalinity while promoting greater nutrient accumulation. Correlation analysis showed that plant
nutrient concentrations, particularly nitrogen and potassium, were more closely associated with
growth and biomass accumulation than total soil nutrient levels. These findings demonstrate that
combining reduced chemical fertilization with vermicompost leachate represents a viable and bio-
economic strategy for sustainable cultivation of C. nutans without compromising yield.
Keywords: Clinacanthus nutans; vermicompost leachate; integrated nutrient management; nutrient use efficiency; sustainable agriculture
Pages 90-99
Hakim, Dani Lukman; Machalett, Bjoern; Wijaya, Arif Supam; & Putra, Merios Gusan
Abstract | Full Text
Abstract
Land degradation caused by unsustainable cultivation practices remains a critical constraint to
agricultural productivity in tropical highland regions. This study investigates the effects of various
integrated agricultural land–use patterns on soil erosion, runoff, and land productivity in a tropical
highland watershed of Southeast Asia. A field experiment and socio–economic survey was conducted
from October 2024 to April 2025 using a Randomized Block Design comprising five treatments: (T1)
cabbage–chili–tobacco, (T2) maize–upland rice, (T3) maize–kidney bean, (T4) coffee–elephant grass,
and (T5) pine forest. Erosion and surface runoff were measured to assess soil loss and water regulation
performance under different land–use types. The results showed that the T1 produced the highest
erosion rate (25.15 t ha–1 yr–1), while T5 recorded the lowest (3.89 t ha–1 yr–1). All systems, however,
exceeded the tolerable erosion threshold for Ultisols(11.21 t ha–1 yr–1), indicating the need for improved
soil conservation measures. Land allocation modeling revealed that an optimal land–use configuration,
comprising 37% mixed agroforestry, 20.8% annual crops, and 5.7% grasslands, maximized income
while minimizing soil loss. The findings underscore the importance of diversified and conservation–
oriented land–use planning for achieving sustainable agricultural production and watershed resilience
in tropical highland environments.
Keywords: Soil erosion, integrated farming systems, land–use optimization, watershed management, tropical highlands
Pages 100-119
Nursyazni Abdul Rahim; Daljit Singh Karam; Nurul Hidayah Adenan; Dzarifah Zulperi; Susilawati Kasim; Arifin Abdu; & Murugesu M Narasimha
Abstract | Full Text
Abstract
Spent coffee grounds (SCG) represent a growing waste stream with potential for agricultural
valorization, yet their direct soil application is limited by phytotoxic compounds. This study evaluated
the physico-chemical properties and agronomic effectiveness of SCG compost enriched with biochar
and agricultural residues. Four formulations were composted over 91 days: SCG (Heap 1), SCG with
rice husk biochar (Heap 2), SCG with rice husk biochar and empty fruit bunches (Heap 3), and SCG
with palm kernel shell biochar (Heap 4). Temperature, pH, weight loss, lead (Pb) concentration,
germination index (GI), root elongation, and leaf cell wall thickness of maize (Zea mays L.) were
assessed. Heap 3 exhibited the highest thermophilic activity (p = 0.0029 vs. Heap 1) and GI (95.3%).
Biochar-amended treatments stabilized pH near neutrality, whereas SCG alone remained acidic.
Linear regression confirmed significant weight reduction across all treatments (p < 0.001), with
biochar enhancing mass retention. Lead (Pb) concentrations declined to undetectable levels by day 91
in all heaps. Root elongation was significantly greater in biochar-amended composts (6.46–7.50 cm)
compared to SCG alone (4.48 cm; p < 0.001). Transmission electron microscopy revealed that Heap 4
significantly increased maize leaf cell wall thickness relative to the control (mean difference = 0.201
µm, p = 0.001). These findings demonstrate that biochar-enriched SCG composting produces mature,
non-phytotoxic amendments that enhance maize seedling vigor and leaf structural development.
Keywords: Biochar, spent coffee grounds, palm kernel shell biochar, empty fruit bunches
Pages 120-131
Vu, Quyen Thi; Nguyen, Giang Vu Huong; & Nguyen, Anh Luong Lam
Abstract | Full Text
Abstract
The mismanagement of dairy cattle manure under tropical conditions contributes to odor pollution,
nutrient loss, and environmental degradation. This study evaluated the individual and combined effects
of wood vinegar (pyroligneous acid), rice husk biochar, and a microbial inoculant comprising Bacillus
licheniformis, Bacillus megaterium, and Lactobacillus acidophilus on odor reduction, composting
kinetics, and the physicochemical and microbiological quality of dairy manure compost in Ho Chi Minh
City, Vietnam. A two-phase experimental design was employed: Phase 1 screened concentration–
response relationships between each amendment and odor intensity using a trained sensory panel (VDI
3882-1 scale, 0–6), and Phase 2 assessed composting performance at the optimal concentrations
identified — wood vinegar at 1:40 (v/v), rice husk biochar at 5% (w/w), and microbial inoculant at 2%
(v/v) – over six weeks. All amendments exhibited concentration-dependent odor reduction. The
combined treatment produced the greatest improvements in organic matter content (56.11%), humic
acid (17.14%), total nitrogen (19.57%), and available phosphorus expressed as P2O5 (11.63%), and
reduced compost maturation time to four weeks compared with six weeks for the unamended control.
The resulting compost met multiple quality thresholds of the Vietnamese national standard for
microbial organic fertilisers (TCVN 7185:2002). These findings demonstrate that this bio-based
amendment system offers a low-cost, scalable approach to sustainable dairy manure management
under tropical conditions.
Keywords: dairy manure; rice husk biochar; wood vinegar; microbial inoculant; odor reduction
Pages 132-141
Noorsuhaila, A.B.; Yaseer Suhaimi, M.; Mazidah, M.; Faridah, M.; & Mohd Hadi Akbar, B.
Abstract | Full Text
Abstract
Cassava (Manihot esculenta Crantz) is an important food crop widely cultivated worldwide and critical
in addressing food security. Optimising plant spacing is essential for improving yield and agronomic
performance in tropical soils. This study evaluated the effect of different plant spacings on growth and
yields using the Markona variety. The experiment consisted of five spacings: T1 (0.50×1.50 m), T2
(0.75×1.50 m), T3 (1.00×1.50 m), T4 (1.25×1.50 m) and T5 (1.50×1.50 m), corresponding to plant
densities of 13 333 plants ha-1, 8 888 plants ha-1, 6 666 plants ha-1, 5 333 plants ha-1 and 4 444 plants
ha-1, respectively. Growth parameters measured included plant height, girth circumference, leaves
number per plant, chlorophyll content (SPAD unit) and root fresh weight yield. Results showed that
plant spacing did not significantly affect plant height, girth circumference, leaves number per plant,
and chlorophyll content. Root yield was greater at plant spacings between 0.75 m and 1.25 m, while
yield was lower at 0.50 m and 1.50 m. The highest yield at 14.59 t/ha was obtained at the 1.25 m spacing
and was significantly higher than T1 and T5. These findings highlight the importance of appropriate
plant spacing in maximising cassava productivity under land constraint and provide useful agronomic
guidance that may be applied to other cassava varieties.
Keywords: Girth circumference, plant spacing, soil, root tuber, yield
Pages 142-153
Nagar Ankita; Jabin Shagufta; Chawla Jyoti; Bir Ritu; & Gandhi Sona
Abstract | Full Text
Abstract
The extensive application of the fungicide zole over last few decades has led to its accumulation as a toxic
residue in agricultural soils and plants. To mitigate its environmental impact and to maintain soil and
plant health, optimization of its dosage is essential. The present study aims to examine the role of
fungicide zole in conjunction with non-polar and neutral amino acid, namely glycine as a soil additive for
the growth of wheat. The experiment is done to explore the growth of wheat plants (Triticum aestivum) at
varying concentration of fungicide zole in terms of seed germination, number of leaves grown, shoot and
root length of the wheat plants while the glycine concentration was held constant. To assess the results on
wheat plant development and nutrient uptake, the experiment includes five different combinations in a
randomized block design (RBD) in three sets of replications. Each set was labelled as S1 (0.1M glycine
and 0% fungicide zole/kg soil), S2 (0.1M glycine and 2% fungicide zole/ kg soil), S3 (0.1M glycine and
4% fungicide zole/kg soil), S4 (0.1M glycine and 6% fungicide zole/kg soil) and S5 (0.1M glycine and 8%
fungicide zole/kg soil). The integrated use of fungicide zole and glycine significantly increases the micro
and macronutrients (N, Na, Mg, Ca, Cr, Fe, Co, Ni, Zn and Cu) of the wheat plant and reduces the
concentration of toxic elements such as cadmium and lead. The number of leaves, seed germination, root
and shoot length show maximum growth at S5. Reduced soil pH and increased electrical conductivity
improve micro and marco-nutrient solubility. improve seed germination, and biomass accumulation. The
finding of this study also indicates that fungicide–amino acid amendments are effective soil conditioners
that improve soil fertility and nutrient uptake in wheat plants, although careful dosage optimization is
necessary to balance crop protection with nutrient bioavailability.
Keywords: Glycine, fungicide zole, wheat plant (Triticum aestivum), Scanning electron microscopy (SEM), Energy Dispersive X-Ray (EDX)
Pages 154-168
Choo, Liza Nuriati L.K.; Zawawi, N.Z.; Ahmed, O.H.; & Sekot, Shamsiah
Abstract | Full Text
Abstract
Insufficient supplies of potassium (K+) in acidic sapric soils impedes pineapple growth and
development, fruit quality attributes, and yield. Clinoptilolite zeolite was incorporated in the MD2
pineapple fertilization programme to evaluate this mineral's effectiveness to improve peat soils' K+
availability and pineapple productivity. Field studies with two MD2 pineapple seasons and laboratory
leaching column experiments were utilized to determine K+ availability in drained sapric soils with the
following treatments: (i) four rates of clinoptilolite at 25, 60, 75, and 100% incorporated with
compound NPK 30:1:32 fertilizer, (ii) standard pineapple fertilization (NPK 30:1:32), and (iii)
untreated soils. Clinoptilolite amended sapric soils improved K+ availability due to the absorption of
K+ ions onto clinoptilolite's lattices and surface frameworks via ion exchange and mass transport. Co-
application of clinoptilolite (100%) and NPK 30:1:32 was more effective in improving peat K+
availability, whereas clinoptilolite fertilization at rates of 25 to 100% improved soil pH (pH: 4.27 –
5.62), increased pineapple K uptake and use efficiency, and yield (2.42–2.66 kg/fruit or 92.0–101.1
t/ha) due to the ability of clinoptilolite to regulate soil exchangeable K+ ions for optimum MD2 plant
uptake compared with standard pineapple fertilization (1.50–1.76 kg/fruit or 57.0–66.9 t/ha). The
recommended clinoptilolite fertilization (25% zeolite + NPK 30:1:32) at the vegetative (3- and 6-
months plant age) and flower induction (9 months plant age) phase provides alternative agronomic
management for pineapple growers to improve peat soil productivity without compromising MD2 yield
production.
Keywords: histosols, natural zeolite, pineapple, potassium, soil conditioners
Pages 169-176
Prusty, Meenakhi; Panda, Narayan; Sahu, Suman G; Mishra, S.S.; Dash, Ashish K; Sahoo, Bignyan R; Barala, Jatiprasad; Patel, Sonali; & Baliarsingh, Anupama
Abstract | Full Text
Abstract
A field experiment was conducted with the objective of enhancing the grain yield of Kharif rice by
substituting soil-applied potassium with nano-potassium. The result revealed that significantly highest
mean total chlorophyll content (3.5 Mg/g), potassium content of leaves (2.61%), root length (16.4 cm)
root volume (20.22 cc), plant height (116 cm), panicle length (22.2cm), panicles/hill (7.1),
spikelet/panicle (119), filled grains/panicle (92) and test weight (25.4gm) was recorded with T5
[100% of soil test dose (STD)+ Twice foliar spray of Nano K at 14 and 28 days after transplanting
(DAT)]. This treatment (T5) also recorded the highest grain yield of 2570 kg/ha and straw yield of
3469 kg/ha as compared to T2 (Nitrogen and phosphorus were applied as per 100% of STD) having
the grain and straw yield of 2494 and 3367 kg/ha respectively. Similarly, the grain and straw yield of
2421 and 3178 kg/ha was recorded with T6 (100% STD for N&P + 75% STD for K + twice foliar
spray of nano-K at 14 and 28 DAT) which was not significantly lower than that of T2. The highest K
content (0.48% in grain, 2.35% in straw) and K uptake (12.22 kg/ha in grain, 81.5 kg/ha in straw)
was recorded with T5 which was not significantly higher than that of T6. The grain and straw yields
were not significantly reduced with T6, indicated that reduction of soil-applied potassium by 25% was
effectively managed with twice foliar application of nano-K without sacrificing yield.
Keywords: Rice, chlorophyll content, foliar application, nano-potassium
Pages 177-191
Khalida Malek Belhamza; Amine Habib Borsali; Mohamed Zouidi; & M’hamed Maatoug
Abstract | Full Text
Abstract
Forests play a central role in terrestrial ecosystems by regulating hydrological and climatic cycles,
preserving biodiversity, and providing environmental ecosystem services. Soil, a limited and non-
renewable resource, is essential for plant production, carbon sequestration, regulation of
biogeochemical cycles, and maintenance of edaphic fertility. However, its degradation, particularly in
arid and semi-arid regions, poses a major environmental challenge, linked to climate change through
a vicious cycle of greenhouse gas emissions and vulnerability to extreme events. Ecological
restoration approaches are emerging as key tools, with forest restoration recognized for curbing
biodiversity loss, enhancing climatic resilience, and improving soil quality. This study evaluates the
potential of spent mushroom substrate (SMC), an organic amendment derived from agricultural waste
rich in nutrients, to restore degraded forest soils. The objective is to analyze its impact on the physical
and chemical properties of the soil, as well as on ecological restoration processes, by comparing the
responses of monospecific and mixed forest stands one year after application. The results show
significant improvements: increases in organic carbon, total nitrogen, organic matter, moisture,
electrical conductivity, water retention, and porosity; decreases in pH, C/N ratio, bulk density, and
permeability. These changes enhance nutrient availability and soil structure, promoting plant
recovery. Spent mushroom substrate appears as a promising tool for the ecological restoration of
degraded forest soils, improving edaphic health and forest resilience, while aligning with circular
economy principles.
Keywords: Soil restoration, monospecific forest, mixed forest, Physico-chemical properties, spent mushroom substrate
Pages 192-205
Sukartono; Jaya, Dori Kusuma; Susilowati, Lolita Endang; Azizah, Isnaniar Rahmatul; Dewi, Intan Lusiana; Kusumo, Bambang Hari; & Yusiharni, Emielda
Abstract | Full Text
Abstract
Open-field burning of agricultural residues remains a common practice in Indonesian farming systems
and contributes to environmental degradation. Converting these biomasses into biochar using a simple
instrument is likely to benefit smallholder farmers in semi-arid tropical regions. This study
characterises biochar produced in Lombok Island from four locally abundant feedstocks (Rice Husk
(RH), Corn Cob (CC), Coconut Fibre (CF), Tobacco Stem (TS)). Analysis by SEM, BET, FTIR, and
XRD shows these biochars have different properties as a function of feedstock type and pyrolysis
conditions. SEM revealed varying porous structures; CC had the widest pores. BET showed that TS
biochar had the highest surface area (17.08 m2 g-1) and the longest pyrolysis time (59 min), whereas
CC had the lowest surface area (5.74 m2 g-1) and the shortest pyrolysis time (14 min). FTIR analysis
revealed that all biochars share key functional groups (-OH, C=C, C-H) but also exhibit feedstock-
specific traits. RH and CC are rich in silica (Si-O-Si), CF contains complex oxygen groups, and TS has
a stable aromatic structure. These findings suggest that RH biochar is suited for inorganic adsorption
and as a silica soil amendment, CC for organic molecule adsorption, and CF/TS biochars for long-term
soil carbon sinks and CEC enhancement. This work provides practical guidance for selecting biochar
types for agricultural and environmental applications.
Keywords: BET, biochar, FTIR, local feedstocks, simple instrument
Pages 206-214
Muhamad Noor, Siti Hazwani; Ariffin, Mohd Rizal; Abdul Sukor, Arina Shairah; Zaibon, Syaharuddin; Amran, Athirah; Md Nor, Nur Atikah; & Mohd Razib, Annur
Abstract | Full Text
Abstract
Durian (Durio zibethinus), often referred to as the “King of Fruits” in Asia, holds significant economic
potential for farmers and exporters. To meet the high demand for durian, it is essential to improve the
methods and challenges of cultivating durian seedlings during the nursery stage. This study aims to
determine the optimal NPK fertilizer rates for durian seedlings, supporting vegetative growth, and
assessing their impact on soil properties. The research was carried out at the greenhouse of Ladang
15, Faculty of Agriculture, UPM, from April to September 2022. Newly grafted durian seedlings of two
cultivars, IOI (D168) and Musang King (D197), were transplanted into polybags filled with limed
topsoil. Four NPK Fertilizer treatments were tested: T1 (2.5 g), T2 (5 g), T3 (7.5 g), and T4 (10 g). The
experimental units were arranged in a Randomized Complete Block Design (RCBD) with four
replications. Data was statistically analyzed using the Statistical Analysis System (SAS) software. The
results indicated that the varying NPK rates affected the growth performance of both IOI and Musang
King seedlings.
Keywords: Durian, soil fertility, growth performance, nursery stage
Pages 215-224
Rizky Amnah; Darnetty Darnetty; Syafrimen Yasin; Amsar Maulana; & Herviyanti Herviyanti
Abstract | Full Text
Abstract
Sidimpuan salak frond waste (SSFW), which has not been optimally utilized, has the potential to become
an environmentally friendly local ameliorant material to increase the productivity of smallholder
Sidimpuan salak (Salacca sumatrana Becc.) plantations. This study has examined the characteristics
of SSFW and its effect on changes in surface charge and nutrient composition of Inceptisol on
Sidimpuan salak (Salacca sumatrana Becc.) plantation ameliorated with SSFW. This study has used a
completely randomized design with three replications and five treatment levels (SSFW doses: 0, 20, 40,
60, and 80 tons ha-1). The characteristics of SSFW have moisture (14.15%), volatile matter (84.40%),
ash (17.81%), fixed carbon (0.63%), pH (7.80), electrical conductivity (0.88 dS m-1), CEC [26.60
cmol(+) kg-1] and composition of macro (20.85% C, 0.53% N, 0.52% P and 0.53% K) and micro
(12.97% Ca, 3.34% Mg, 4.72% S, 0.47% Mn, 11.61% Fe and others) nutrients and has active groups
such as phenol and carboxyl. The application of 40 tons ha-1 of SSFW significantly increased the surface
charge (pH H2O, EC, and CEC) by 0.3, 0.06 ds m-1, and 1.60 cmol(+) kg-1, respectively. Meanwhile, it
is also significant to increase the availability of nutrients (organic C, total N, available P, and K-exch)
by 0.45% C, 0.15% N, 19.64 ppm, and 0.56 cmol(+) kg-1 K, respectively. The chemical and nutrient
characteristics of SSFW and the application of 40 tons ha-1 SSFW have significantly improved soil
quality (Inceptisols) and support sustainable productivity on smallholder Sidimpuan salak (Salacca
Sumatrana Becc.) plantation land.
Keywords: Amelioration, Inceptisols, salak frond waste, Sidimpuan salak, smallholder plantation land
Pages 225-233
Nur Liyana Iskandar; Faridah Manaf; Zurin Aida Jamilan; & Mohamad Fakhri Musa
Abstract | Full Text
Abstract
Soil-borne diseases represent a major constraint to chilli (Capsicum spp.) production due to the
persistence of pathogenic fungi in soil and their strong association with soil health conditions.
Conventional management practices relies on chemical fungicides often provide limited control of
soil-resident inoculum and may negatively affect soil biological activity. Biological control agents
with the ability to suppress soil-borne pathogens are increasingly explored as sustainable
alternatives. Among these, Trichoderma are widely recognized for their antagonistic activity against
a range of soil-borne fungal pathogens. The present study aimed to evaluate the antagonistic potential
of T. asperellum against selected soil-borne fungal pathogens of chilli. Antagonistic activity was
assessed under laboratory conditions using dual culture assays to determine the inhibitory effects of
T. asperellum isolates on pathogen growth. By using soil dilution plating method, T. asperellum was
isolated from the healthy chilli rhizosphere cultivated in MARDI Organic Farm and fungal pathogens
were obtained from the infected chilli plants. The results demonstrated strong antagonistic activity of
T. asperellum with inhibition of pathogen growth ranging from 65.5% to 81.1%. These findings
confirm the role of T. asperellum as a beneficial soil fungus and highlight its potential as a biocontrol
for managing chilli diseases.
Keywords: Trichoderma, Soil-borne pathogens, chilli, dual culture assay
Pages 242-258
Alomar, Rahaf; Jena, Dinabandhu; Dash, Ashish K.; Mohanty, Samanyita; & Barala, Jatiprasad
Abstract | Full Text
Abstract
Soil salinity caused by poor-quality irrigation, climate and drainage limits soil fertility and crop
productivity, while amendments such as organic manure, K, Zn and gypsum can mitigate these effects. A
60-day laboratory incubation study was conducted to assess the impact of saline water and soil amendments
on electrical conductivity and nutrient release pattern. Twenty treatment combinations were evaluated,
consisting of four levels of saline water (SW0: normal water, SW1: 4 dS/m, SW2: 8 dS/m and SW3: 16
dS/m) and five amendments: control (C), organic manure @10 t/ha (OM), KCl @120 kg K/ha (K), ZnSO4
@10 kg Zn/ha (Zn) and gypsum @5 q/ha (Gy). Salinity reduced soil pH by 0.8–2.15% and increased
electrical conductivity by 36–146% over 60 days. Organic manure and Zn increased organic carbon by
62% and 71% respectively; K enhanced available K by 34–52% in SW3K and gypsum improved Ca by 75%
at 45 days. All amendments reduced Na, with K and gypsum showing the highest reductions (8%).
Incubation time increased soil pH, organic carbon and nutrient availability. Potassium under high salinity
was most effective in mitigating stress, while Zn and organic manure enhanced organic carbon, showing
amendments can alleviate salinity impacts and improve soil fertility.
Keywords: Salinity, organic matter, potassium, zinc, gypsum
Pages 259-269
Grace Esshalina Misak; Lissa Cressenta Laizen; Alvira Avida Raphael; Sukaibin Sumail; Jacqueline Joseph; & Farnidah Jasni
Abstract | Full Text
Abstract
Ultramafic landscapes are ecologically unique yet remain underrepresented in tropical soil carbon
research, particularly under transitioning land-use systems. This study assessed soil organic carbon
(SOC) sequestration in Pahu Agromining Trial Farm, Kg. Pahu Pinawantai, Ranau, Sabah, a living
laboratory cultivated with Phyllanthus rufuschaneyi, by comparing two types of land use; an early-
stage agroforestry system with an adjacent ultramafic forest. Three inventory plots, 10m × 10m were
established in each land-use system, and soil composite samples were collected to evaluate SOC stocks
and associated physicochemical properties. For the determination of total organic carbon (TOC), total
nitrogen (TN), and total phosphorus (TP), samples were outsourced to the Chemistry Section of the
Forest Research Centre, Sandakan. Meanwhile, soil organic matter (SOM), bulk density, and soil pH
were analysed at the Kompleks Sains dan Teknologi (KomSAT) Laboratory, Universiti Teknologi
MARA Sabah Branch. Results showed that mean SOC stocks were higher in the early-stage agroforestry
system (42.20 ± 15.25) than in the ultramafic forest (30.94 ± 9.44). Nonetheless, the difference was not
statistically significant (p > 0.05), indicate that SOC stocks in the early-stage agroforestry system are
comparable to those of the adjacent ultramafic forest, thus suggesting that soil carbon levels have not
changed substantially at this early transitional stage. Correlation analyses indicated moderate to
strong positive relationship (r= 0.300 – 0.900) between SOC and selected soil physicochemical
properties. Overall, the findings suggest that early-stage agroforestry systems have the potential to
function as complementary soil carbon sinks in ultramafic environments. This study highlights the
potential of agroforestry as a nature-based solution that not only supports climate change mitigation
in tropical ultramafic landscapes but also presents promising opportunities for participation in
emerging nature-based carbon credit markets in Sabah through enhanced carbon sequestration and
sustainable land management practices.
Keywords: Agroforestry, hyperaccumulators, land-use, soil organic carbon, Ultramafic soils
Pages 270-288
Zafirah Zainal Abidin; Muhammad Zhafiran Zamri; Noorsuhaila Abu Bakar; & Mohd Hadi Akbar Basri
Abstract | Full Text
Abstract
The oil palm industry generates substantial quantities of biomass waste, particularly palm oil
mill effluent (POME), which poses significant environmental challenges due to its high
organic load and pollutant content. Untreated POME may contribute to soil clogging and
water pollution, whereas treated POME has potential as a nutrient-rich organic amendment
capable of reducing dependence on inorganic fertilizers. However, the effectiveness of
integrating treated POME with chemical fertilizers in vegetable production systems remains
poorly understood.This study evaluated the combined effects of treated POME and NPK
fertilizer on the growth, yield, postharvest quality, and soil chemical properties of cucumber
(Cucumis sativus L.) using a randomized complete block design with nine treatments.
Integrated POME–NPK applications significantly influenced stem diameter, root
development, fruit yield, and postharvest weight loss. Notably, the treatment consisting of 3 g NPK combined with 300 ml POME achieved comparable performance to the conventional
farmer practice of 6 g NPK while reducing inorganic fertilizer input by 50%. These findings
demonstrate that treated POME can partially substitute inorganic fertilizers without
compromising crop performance, highlighting its potential as a sustainable nutrient
management strategy for cucumber production.
Keywords: Cucumis sativus L., palm oil mill effluent, NPK fertilizer
Pages 299-317
Cassandra Sarah David; Latifah Omar; Mohd Hasyrin Hassan; Kavinraj Krishnan; & Osumanu Haruna Ahmed
Abstract | Full Text
Abstract
Bekenu Series has low pH, poor organic matter content, and limited basic nutrients which hindered
crop production. This study evaluated the effects of combining urea, Egyptian rock phosphate (ERP),
and BSFL frass on dry matter, nitrogen (N) uptake, phosphorus (P) uptake, and nutrient use efficiency.
A pot experiment was conducted using maize grown in Bekenu Series soil under seven different
fertilizers treatments (UP, UP1F1, UP2F1, UP1F2, UP2F2, UP1F3, and UP2F3). Plants were harvested at
the tasseling stage. Urea, ERP and BSFL frass combination (UP1F1, UP2F1, UP1F2, UP2F2, UP1F3, and
UP2F3) significantly improved dry matter of maize with the range of 56.9 g plant-1 to 63.2 g plant-1
compared with UP (11.5 g plant-1). Greater N uptake (0.76 to 0.88 g plant-1) was achieved in all
combined fertilizers with BSFL frass application compared with fertilizer only (UP) (0.17 g plant-1).
Higher P uptake recorded in maize fertilized with UP2F1, UP1F2, UP2F2, UP1F3, and UP2F3 (0.41 to
0.45 g plant-1), compared with UP (0.09 g plant-1) and UP1F1 (0.23 g plant-1). The UP treatment did
not improve nutrient use efficiency, with lower N use efficiency (9.79%) than combined fertilizers and
BSFL frass. In contrast, treatments UP2F1, UP1F2, UP2F2, UP1F3, and UP2F3 recorded higher N use
efficiency, ranging from 15% to 20%. Similarly, P use efficiency was higher in the combined fertilizer
with BSFL frass treatments (5% to 7%) than those of fertilizer only treatment (2%). UP2F1, UP2F2, and
UP2F3 showed best improvements in N and P uptake and use efficiency.
Keywords: Acidification, frass, functional groups, nutrient use efficiency, organic fertilizer
Pages 318-325
Nwokocha Amarachi G.; Okorie Chinedu N.; Idris Sani; & Fagbola O.
Abstract | Full Text
Abstract
The persistence and bioavailability of insecticides are influenced by environmental factors, soil
properties and ageing factor, which can affect the residual efficacy of soil-applied termiticides. This
study assessed the residual efficacy of Bithor® Dual Action against Coptotermes gestroi, in mineral
and peat soils under different temperatures (30 and 40 °C), soil pH conditions (acidic, neutral, and
alkaline), and ageing periods (1 day, 1 week, 1 month, 3 months, and 6 months). Treated soils were
tested to a no-choice laboratory bioassay with termite mortality monitored over 7-days. Probit analysis
was used to estimate lethal times (LT50) while a four-way General Linear Model (GLM) was used to
analyse the effects of ageing time, temperature, soil type, and pH. Termite mortality declined
progressively with increasing ageing period, as demonstrated by increased LT50 values. Mineral soil
consistently killed termites faster than peat soil, however higher temperatures (40 °C) lowered residual
efficacy than at 30 °C. The GLM analysis showed that ageing time, temperature, and soil type
significantly affected termite mortality (p < 0.001), whereas soil pH had no significant effect (p > 0.05).
Significant interaction effects among environmental factors revealed that residual efficacy was
influenced was dependent on their combined effects. Overall, ageing time and soil type are the major
factors influencing residual performance of Bithor® Dual Action in soil. Reduced efficacy in peat soil
may be attributed to decreased bioavailability associated with its high organic matter content. These
findings provide useful information for optimizing soil termiticide application strategies in tropical
environments.
Keywords: Bifenthrin, Imidacloprid, residual efficacy, soil ageing, Coptotermes gestroi
Pages 326-335
Kangheswaraan Kannan; Tee Tuan Poy; Mohd Firdaus Mohd Anuar; & Sarah Baharudin
Abstract | Full Text
Abstract
The accumulation of agricultural wastes such as crop residues and animal manures in Malaysia are
increasingly generated and are often inadequately managed, causing environmental hazards. However,
these wastes have significant economic value and potential to be transformed into useful products like
biochar within a circular economy framework. This study evaluated physicochemical properties of six
selected agricultural wastes between crop-based residue i.e. rice husk, coconut husk, oil palm empty
fruit bunch, and animal-based manure i.e. cow manure, goat manure, and chicken manure by producing
biochar at 500 °C for 30 minutes under laboratory conditions. Biochar yields ranged from 32% to 50%.
Manure-derived biochars generally exhibited higher yields. Ash content differed from 9% to 55% and
was higher in manure-derived biochar due to greater mineral composition. Crop residue biochar
showed higher moisture content, higher total carbon, more developed pore structures in SEM, and
lower H:C molar ratios (<0.7) which demonstrating greater stability. All biochar except rice husk
biochar featured alkaline properties (pH 7.51–9.46). Animal manure biochar is nutrient-rich in macro-
and micronutrients, with chicken manure biochar showing the highest nutrient levels (e.g., P – 2.44%,
K – 5.43%, Ca – 9.20%) but also a very high electrical conductivity (23.12 dS/m) and poor pore
structure. This comparative study provides fresh insights for optimizing feedstock selection in scalable
and cost-effective biochar production.
Keywords: Biochar, lab-scale pyrolysis, animal manure, physico-chemical analysis
Pages 336-347
Ali Hassan, N.A.A.; Zin Zawawi., N.; Anuar, N.A.; Raja Abdullah, R.Z.; Mohamad Nizar, M.M.; Rabidi, M.N.; & Ahmad Rashdi, A.
Abstract | Full Text
Abstract
Soil microbial functional diversity is critical to soil health and agricultural sustainability. This study
evaluated the short-term responses of soil microbial functional diversity to organic amendments in
paddy fields at IADA Kemasin Semerak. Soil samples were collected at three stages: before amendment
application (T1), four weeks post-application (T2), and after paddy harvest (T3). The effects of organic
amendments on soil chemical properties were evaluated by measuring soil pH, electrical conductivity,
total nitrogen, soil organic carbon, and available phosphorus. Microbial functional diversity was
assessed using the Biolog Ecoplate system, focusing on microbial activity, species richness, species
evenness, and Shannon diversity index. Substrate utilization was further classified into five functional
guilds. The application of organic amendments significantly increased soil pH and the availability of
phosphorus. Results indicated a significant increase in microbial activity and species richness following
amendment application, while no significant differences were observed in species evenness or diversity
index across sampling stages. Organic amendment application enhanced microbial substrate utilization
across all functional groups compared with the pre-amendment treatment. The greatest increases were
observed for carboxylic and acetic acids (133.8%) and amino acids (127.0%). Microbial substrate
utilization remained elevated following paddy harvest, with carbohydrates showing the highest increase
(149.9%) relative to the pre-amendment condition. The application of organic amendments and
following paddy harvest significantly increased utilization of L-arginine, L-asparagine, L
phenylalanine, pyruvic acid methyl ester, D-mannitol, N-acetyl-D-glucosamine, D-cellobiose, D
galacturonic acid, 4-hydroxybenzoic acid, γ-hydroxybutyric acid, D-malic acid, Tween 40, and Tween
80. In conclusion, organic amendments exert positive short-term effects on soil microbial functional
diversity, particularly by enhancing microbial activity, species richness, and substrate utilization
capacity.
Keywords: microbial activity, microbial diversity, species richness, Ecoplate, paddy
Pages 348-359
Mohd Khairil Jamahuri; Wan Mohd Razi Idris; Zulfahmi Ali Rahman; Mohd Hadi Akbar Basri; and Wan Abd Halim Wan Ilias
Abstract | Full Text
Abstract
Tropical peat soils are extensively distributed across Southeast Asia and support large-scale oil palm
cultivation, however their high porosity, low bulk density and poor load-bearing capacity limit root
anchorage, palm stability and long-term productivity. These physical constraints contribute to impaired
root development, reduced nutrient uptake efficiency and yield decline presenting major challenges for
sustainable peatland management. This study evaluated the effectiveness of soil mounding as an
agronomic intervention to improve yield recovery, nutrient status and root development of oil palm
cultivated on tropical peat soils in Malaysia. Soil mounding was established around the palm base using
elevated soil structures measuring 1 m (height) × 2 m (width) × 2 m (length) to enhance mechanical
soil support, root anchorage and rhizosphere conditions. Two-way ANOVA demonstrated significant
effects of treatment, year and treatment × year interaction on yield-related parameters (p < 0.001).
Following soil mounding application, fresh fruit bunch (FFB) yield increased by 128.4%, from 9.92 to
23.30 t ha-1, within 2–3 years. Average bunch weight increased by 134.9% (11.51 to 23.35 kg) while
bunch number per palm increased by 200.0% (3 to 9 bunches palm-1). Tukey’s HSD analysis confirmed
significant differences between soil-mounded palms and untreated controls (p ≤ 0.05) indicating
enhanced yield recovery following soil modification. Furthermore, soil mounding significantly
improved leaf nutrient concentrations and root growth characteristics suggesting enhanced nutrient
acquisition and root system establishment. Overall, soil mounding effectively mitigates key physical
limitations of tropical peat soils by improving root-zone stability, nutrient utilization and reproductive
performance. This approach provides a sustainable management strategy for restoring oil palm
productivity and enhancing yield resilience in peatland cultivation systems.
Keywords: Oil palm, peat soil, soil mounding, nutrient status, FFB yield